This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lin, B. Articles by Pancrazio, J. J Search for Related Content PubMed PubMed Citation Articles by Lin, B. Articles by Pancrazio, J. J Social Bookmarking                   What's this?

Identification of target genes responsive to JP-8 exposure in the rat central nervous system

Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, Washington, District of Columbia 20375, USA

Glenn D Ritchie

Geo-Centers, Inc., Wright-Patterson Air Force Base (WPAFB), Ohio 45433, USA

John Rossi, III

Naval Health Research Center/Toxicology Detachment, Wright-Patterson Air Force Base (WPAFB), Ohio 45433, USA

Joseph J Pancrazio

Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, Washington, District of Columbia 20375, USA, jxp{at}cbmse.nrl.navy.mil

Concern for the health risk associated with occupational exposure to jet fuel has emerged in the Department of Defense. Jet propulsion fuel-8 (JP-8) is the fuel used in most US and North Atlantic Treaty Organization (NATO) jet aircraft, and will be the predominant fuel both for military land vehicles and aircraft into the twenty-first century. JP-8 exhibits reduced volatility and lower benzene content as compared to JP-4, the predominant military aircraft fuel before 1992, possibly suggesting greater occupational exposure safety. However, the higher rates of occupational exposure through fueling and maintenance of increasingly larger numbers of aircraft/vehicles raise concerns with respect to toxicity. Clinical studies of workers experiencing long-term exposure to certain jet fuels demonstrated deficits in CNS function, including fatigue, neurobehavioral changes, psychiatric disorders, and abnormal electroencephalogram (EEG). In the present study, cDNA nylon arrays (Atlas Rat 1.2 Array, Clontech Laboratories, Palo Alto, CA) were utilized to measure changes in gene expression in whole brain tissue of rats exposed repeatedly to JP-8, under conditions that simulated possible real-world occupational exposure (6 h/day for 91 days) to JP-8 vapor at 1000 mg/m³. Gene expression analysis of the exposure group compared to the control group revealed a modulation of several genes, including glutathione S-transferase Yb2 subunit (GST Yb2); cytochrome P450 IIIA1 (CYP3A1); glucose-dependent insulinotropic peptide (GIP); a1-proteinase inhibitor (a1-AT); polyubiquitin; GABA transporter 3 (GAT-3); and plasma membrane Ca²⁺-transporting ATPase (brain isoform 2) (PMCA2). The implications of these vapor-induced changes in gene expression are discussed.

Key Words: 1-proteinase inhibitor (1-AT) • cDNA array • central nervous system • cytochrome P450 IIIA1 (CYP3A1) • GABA transporter 3 (GAT-3) • gene expression • glucose-dependent insulinotropic peptide (GIP) • glutathione S-transferase Yb2 subunit (GST Yb2) • jet propulsion fuel-8 • neurobehavioral deficits • neurotoxicity • plasma membrane Ca2+-transporting ATPase (brain isoform 2) • polyubiquitin

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. N. McDougal, C. M. Garrett, C. M. Amato, and S. J. Berberich Effects of Brief Cutaneous JP-8 Jet Fuel Exposures on Time Course of Gene Expression in the Epidermis Toxicol. Sci., February 1, 2007; 95(2): 495 - 510. [Abstract] [Full Text] [PDF]

				L. A. Espinoza, M. Valikhani, M. J. Cossio, T. Carr, M. Jung, J. Hyde, M. L. Witten, and M. E. Smulson Altered Expression of {gamma}-Synuclein and Detoxification-Related Genes in Lungs of Rats Exposed to JP-8 Am. J. Respir. Cell Mol. Biol., March 1, 2005; 32(3): 192 - 200. [Abstract] [Full Text] [PDF]