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Change in the iso-enzyme profiles of urinary N-acetyl-b-D-glucosoaminidase in workers exposed to mercury

Faculty of Chemistry, University of Belgrade, P.O. Box 158, Studentski trg 16, 11001 Belgrade, Yugoslavia, ljmandic{at}helix.chem.bg.ac.yu

Maksimovic Radmila

Krucevac Clinical Center

Acimovic Jelena

Faculty of Chemistry, University of Belgrade, P.O. Box 158, Studentski trg 16, 11001 Belgrade, Yugoslavia

Dobric Dubravka

Faculty of Chemistry, University of Belgrade, P.O. Box 158, Studentski trg 16, 11001 Belgrade, Yugoslavia

The iso-enzyme profiles of urinary N-acetyl-b-D-glucosoaminidase (NAG) were studied in the workers from a chloro-alkaline electrolysis plant that had been continuously exposed to elemental mercury. The same workers then had a work break of four months and were later re-exposed to mercury for another five months. The activities of urinary NAG in workers exposed to mercury before and after the work break and in the control group were 2.099 ^/ 1.03 IU/L; 0.909 ^/ 0.52 IU/L and 1.139 ^/ 0.35 IU/L, respectively. The fraction of the dominant A form activity in the total activity, in the workers exposed to mercury after a work break (85.319 ^/ 5.32%), was almost equal to the fraction of A in the control group (84.649 ^/ 2.75%). The percent fraction of A forms (A and A₂) during continuous exposure was almost equal to the sum of the fractions A and A₂ in the control group and for exposure after the work break. The fraction of the B form activity in the total activity during exposure to elemental mercury for five months after the work break (7.419 ^/ 3.45%) was lower (P B ^/ 0.1) than the fraction of the B form in the control group (8.629 ^/ 2.19%). The decrease in the fraction of the B form compared with the control group was more significant (P B ^/ 0.001) in the case of continuously exposed workers (5.259 ^/ 2.55%). Beside the major A and B iso-enzymes of NAG, the A₂form was also isolated, and its fraction in the control group (6.739 ^/ 2.15%) was not negligible. For workers exposed to mercury for five months, it was 7.289 ^/ 3.52%. It is concluded that mercury affected the increased exocytosis of iso-enzyme A and the inhibition of B iso-enzyme of NAG. The increase in activity of urinary NAG in subjects exposed to mercury was a consequence of the increased excretion of A form. It was established also that the four months work break was sufficient for the repair of renal damages that occurred during continuous exposure, and which were monitored by the activity of NAG. Thus, the effects of mercury seem to have been reversible.

Key Words: N-acetyl-b-D-glucosaminidase • elemental mercury • iso-enzymes profiles

Toxicology and Industrial Health, Vol. 18, No. 5, 207-217 (2002)
DOI: 10.1191/0748233702th144oa


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