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Ratios of N-(2,3,4-trihydroxybutyl) valine and N-(2-hydroxy-3-butenyl) valine formed hemoglobin adducts in female mice inhalation exposure with 1,3-butadiene

Department of Environmental Education, Kongju National University, Kongju, Chungnam-do, South Korea, ejhl{at}kongju.ac.kr

Hee Sook Kang

Department of Social Welfare, Kongju National University, Kongju, Chungnam-do, South Korea

Don-Hee Han

Department of Occupational Health and Safety Engineering, Inje University, Gimhae, Gyeongnam-do, South Korea

1,3-Butadiene (BD) is a known rodent and probable human carcinogen (IARC, group 2A) or ‘known to be a human carcinogen’ (Department of Health and Human Services, 2000). Exposure to BD can occur either via petrochemical products or through the general environment. Adducts can be used as biomarkers for biological monitoring of carcinogen exposure. This study investigated the hemoglobin adducts in blood after inhalation exposure to BD in ICR female mice for three weeks (5 h/day-5 days/week). During the inhalation exposure, the body weights of mice were significantly lower from day 9 onward for the 500 ppm BD group and from day 4 onward for the 1000 ppm BD group. On the 1st, 2nd and 3rd weeks after inhalation exposure, the concentrations of HB Val adducts were 1.8, 3.7 and 6.2 pmol/mg globin for the 500 ppm BD group, and 5.7, 7.4 and 16.0 pmol/mg globin for the 1000 ppm BD group. The concentrations of THB Val adducts were 32.0, 42.0 and 55.0 pmol/mg globin for the 500 ppm BD group, and 67.8, 72.7 and 83.5 pmol/mg globin for the 1000 ppm BD group. Their defined ratios were higher at the earlier exposure period and at the lower concentration. They were 17.8, 11.4 and 8.87 for the 500 ppm BD group, and 11.9, 9.8 and 5.2 for the 1000 ppm BD group, on the 1st, 2nd and 3rd weeks after inhalation exposure. THB Val and HB Val adducts appear to be the important hemoglobin adducts for monitoring BD exposure, with the latter being a more predictable biomarker than the former.

Key Words: 1,3-butadiene (BD) • biomarker • GC/MS • (N-2-hydroxy-3-butenyl)valine (HB Val adduct) • (N-2,3,4-trihydroxy-butyl)valine (THB Val adduct)

Toxicology and Industrial Health, Vol. 21, No. 10, 15-20 (2005)
DOI: 10.1191/0748233705th210oa


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