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Toxicology and Industrial Health, Vol. 21, No. 5-6, 147-154 (2005)
DOI: 10.1191/0748233705th223oa

In vitro cytotoxicity testing of airborne formaldehyde collected in serum-free culture media

S Bakand

Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia, bakand{at}student.unsw.edu.au

A Hayes

Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia

C Winder

Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia

C Khalil

Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia

B Markovic

Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia

The purpose of this study was to identify a suitable sampling model for on-site toxicity assessment of soluble air contaminants such as formaldehyde, a well known industrial and indoor air contaminant. The in vitro cytotoxicity of formaldehyde, the selected model for soluble air contaminants, was studied using the MTS (tetrazolium salt) assay in two carcinoma cell lines, A549 epithelial lung and HepG2 hepatocarcinoma, and in skin fibroblasts. The cytotoxic effects of airborne formaldehyde were evaluated using test atmospheres in concentrations below 10 ppm (12.3 mg/m3), generated by a dynamic diffusion method and bubbled (0.3 L/min) through serum-free culture media for one or four hours. Human cells were treated with formaldehyde air samples, and cell viability was determined after four hours incubation. In parallel, the concentration of airborne formaldehyde was monitored, using the 3500 NIOSH method. Cell viability of the HepG2 cells exposed to formaldehyde air samples (8.75 ppm-4 h) was reduced to less than 50% (31.69/1.24%). The HepG2 cell lines were found to be more sensitive (IC50=103.799/23.55 mg/L) to formaldehyde than both A549 cell lines (IC50=198.369/9.54 mg/L) and skin fibroblasts (IC50=196.689/36.73 mg/L) (PB/0.01). An average of 96.8% was determined for collection efficiency of formaldehyde in serum-free culture media. The results of this study suggest that absorption of soluble air contaminants, such as formaldehyde, in serum-free culture media can be used as a suitable sampling model for on-site toxicity assessments.

Key Words: air contaminants • cytotoxicity • formaldehyde • in vitro toxicity • MTS (tetrazolium salt) assay


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