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Photoirradiation of representative polycyclic aromatic hydrocarbons and twelve isomeric methylbenz[a]anthracene with UVA light: formation of lipid peroxidation

National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA

Ming W Chou

National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA

Jun J Yin

Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA

Paul C Howard

National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA

Hongtao Yu

Department of Chemistry, Jackson State University, Jackson, MS 39217, USA, yu{at}ccaix.jsums.edu

Peter P Fu

National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA

Polycyclic aromatic hydrocarbons (PAHs) are widespread genotoxic environmental pollutants, which require metabolic activation in order to exert biological activities, including mutagenicity and carcinogenicity. Photoactivation is another activation pathway that can lead to PAH genotoxicity. In this paper, we demonstrate that photoirradiation of a series of representative PAHs, with and without bearing a methyl substituent, with UVA light in the presence of methyl linoleate resulted in the formation of methyl linoleate hydroperoxides (a lipid peroxide). The lipid peroxide formation was inhibited by dithiothreitol (DTT) (free radical scavenger), NaN₃ (singlet oxygen and free radical scavenger), and superoxide dismutase (SOD) (superoxide scavenger), but was enhanced by the presence of deuterium oxide (D₂O) (extends singlet oxygen lifetime). These results suggest that photoirradiation of PAHs by UVA light generates reactive oxygen species (ROS), which induce lipid peroxidation.

Key Words: lipid peroxidation • PAHs • photoirradiation • UVA light

Toxicology and Industrial Health, Vol. 22, No. 4, 147-156 (2006)
DOI: 10.1191/0748233706th259oa


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