This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Alpsoy, L Articles by Ikbal, M Search for Related Content PubMed PubMed Citation Articles by Alpsoy, L Articles by Ikbal, M Social Bookmarking                         What's this?

Protective role of vitamins A, C, and E against the genotoxic damage induced by aflatoxin B₁ in cultured human lymphocytes

Faculty of Art and Science, Department of Biology, Fatih University, Istanbul, Turkey

G Agar

Faculty of Art and Science, Department of Biology, Atatürk University, Erzurum, Turkey lalpsoy{at}fatih.edu.tr

M Ikbal

Faculty of Medicine, Department of Medical Genetics, Karadeniz Technical University, Trabzon, Turkey

In this study, we aimed to evaluate the effect of vitamins A, C, and E against aflatoxin B₁ (AFB₁) on blood cultures in relation to induction of sister chromatid exchange (SCE). The results indicated genotoxic and mutagenic damage in cultured human lymphocytes exposed to AFB₁. The results showed that 5 µM concentration of AFB₁ increased SCE. When vitamins A, C, and E were added to AFB₁, the frequency of SCE decreased. These results suggest that vitamins A, C, and E could effectively inhibit AFB₁-induced SCE, which may partially responsible for its mutagenic effect of AFB₁. Besides, the protective effect of vitamins A, C, and E against AFB₁ was increased in a dose-dependent manner (i.e., as the doses increased, their protective effects also increased). There was a significant decrease in the SCE frequency in AFB₁-treated group compared with the groups receiving AFB₁ and also vitamins A, C, and E. The most effective concentration was 100 microM vitamin C, and the lowest effective concentration was 0.5 microM vitamin A. Vitamin C has the most effective concentration of 100 µM, and vitamin A has the lowest effective concentration of 0.5 µM. The order of the decreasing effect of the SCE frequency of vitamins was as follows: vitamin C > vitamin E > vitamin A.

Key Words: aflatoxin B1 • genotoxicity • sister chromatid exchange • vitamins A • C • and E

Toxicology and Industrial Health, Vol. 25, No. 3, 183-188 (2009)
DOI: 10.1177/0748233709106068


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?