This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kadioglu, E Articles by Karakaya, A. Search for Related Content PubMed PubMed Citation Articles by Kadioglu, E Articles by Karakaya, A. Social Bookmarking                         What's this?

Determination of DNA damage by alkaline halo and comet assay in patients under sevoflurane anesthesia

Department of Toxicology, Gazi University, Faculty of Pharmacy, Ankara, Turkey

S Sardas

Department of Toxicology, Marmara University, Faculty of Pharmacy, Istanbul, Turkey semrasardas{at}gmail.com

S Erturk

Department of Anaesthesiology and Intensive Care, Ankara University, Faculty of Medicine, Ankara, Turkey

O Ozatamer

Department of Anaesthesiology and Intensive Care, Ankara University, Faculty of Medicine, Ankara, Turkey

AE Karakaya

Department of Toxicology, Gazi University, Faculty of Pharmacy, Ankara, Turkey

In this study, we report data on the possible genotoxic effect of inhalation anesthetic sevoflurane (SVF) by comparing two techniques, comet and alkaline halo assay, in peripheral blood lymphocytes (PBL) of patients before, during, and after anesthesia and in controls. DNA single strand breaks were detected in PBL of malignant breast cancer diagnosed patients (stage II–III), who were undergoing mastectomy. Blood samples were taken before the induction of anesthesia, at 120 min of SVF anesthesia, and on the postoperative fifth day. The nuclear spreading factor (NSF) for each cell was assessed by alkaline halo assay, and the total comet score (TCS) was evaluated by comet assay. A statistically significant increase (P < 0.0001) was observed in the mean NSF at 120 min of anesthesia (38.24 ± 14.14) as compared with samples before anesthesia (12.33 ± 6.14), and the mean NSF was significantly decreased after the postoperative fifth day (17.89 ± 9.44). Similar results were obtained by the comet assay with significant increase (P < 0.0001) in DNA damage at 120 min of anesthesia (79.66 ± 15.28) as compared with samples before anesthesia (36.30 ± 11.39). The DNA damage was almost with the preoperative damage rates after the fifth day of anesthesia (43.40 ± 12.19). In conclusion, the study points out a reversible genotoxic effect of SVF and the similar DNA damage levels obtained by comet and alkaline halo assay indicate that although halo assay has a completely different principle, it can conveniently be utilized for the assessment of DNA single strand breakage in individual mammalian cells with its experimental advantages.

Key Words: alkaline halo assay • comet assay • DNA damage • sevoflurane anesthesia • single strand breaks

Toxicology and Industrial Health, Vol. 25, No. 3, 205-212 (2009)
DOI: 10.1177/0748233709106445


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?