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Beta-carotene prevents ozone-induced proinflammatory markers in murine skin

Department of Biomedical Sciences, University of Siena, via Aldo Moro, Siena, Italy; Department of Food and Nutrition, Kyung Hee University, Seoul, South Korea valacchi8{at}unisi.it

A Pecorelli

Department of Physiology, University of Siena, via Aldo Moro, Siena, Italy

M Mencarelli

Department of Physiology, University of Siena, via Aldo Moro, Siena, Italy

E Maioli

Department of Physiology, University of Siena, via Aldo Moro, Siena, Italy

PA Davis

Department of Nutrition, University of California Davis, Davis, USA

Beta-carotene has been thought to protect against oxidative stress generated by ultraviolet radiation and thus prevents skin cancer and skin aging (Biesalski and Obermueller-Jevic, 2001). However, nothing is known about its potential effects against other environmental sources of oxidative stress such as ozone (O₃) in skin. Intake of oral β-carotene supplements before exposure to sunlight (and thus inevitably also to O₃) has been recommended on a population-wide basis. However, although some studies have shown β-carotene as providing skin protection as an antioxidant, other studies using skin cells in culture have shown that β-carotene may have unexpected prooxidant properties (Obermüller-Jevic, et al., 2001). Given this, there is an ongoing debate regarding the protective or potentially harmful role(s) of β-carotene in human skin. In this study, the effect of β-carotene on ozone’s effects on the skin of hairless mice was assessed. After feeding a diet supplemented with 0.5% β-carotene for 1 month, mice were subjected to O₃ exposure (0.8 ppm 6 h/day; 7 days) and the induction of proinflammatory markers such as tumor necrosis factor- (TNF), macrophage inflammatory protein 2 (MIP2), and inducible nitric oxide synthase (iNOS), and markers of oxidative stress, heme-oxygenase-1 (HO-1), were quantitated. The data showed that β-carotene downregulated the induction of TNF, MIP2, iNOS, and HO-1 in response to O₃. We conclude that β-carotene provides protection against O₃-induced skin oxidative stress in vivo, which is consistent with a protective role for β-carotene in the skin.

Key Words: β-carotene • HO-1 • inflammation • ozone

Toxicology and Industrial Health, Vol. 25, No. 4-5, 241-247 (2009)
DOI: 10.1177/0748233709103030


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