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Synergic effects of -tocopherol and β-carotene on tert-butylhydroperoxide-induced HepG2 cell injury

Department of Oriental Medical Food and Nutrition, Semyung University, Seoul, Korea

AJ Kim

Department of Food & Nutrition, Hyejeon College, Choongnam, Korea

M Lee

Department of Food and Nutrition, Sungshin Women’s University, Seoul, Korea mlee{at}sungshin.ac.kr

Oxidative stress produced by the dietary or chemical substrates is one of the major causes of liver cell injury. In this study, we compared the effects of two dietary antioxidants, -tocopherol (-T) and β-carotene (β-C) against tert-butyl hydroperxide (tBHP)-induced oxidative stress in human hepatoma HepG2 cells. Cell proliferation, lipid peroxidation (LPO), cellular lactate dehydrogenase (LDH), [³H]-aflatoxin B₁(AFB₁)-DNA adduct formation, and cytochrome P450 2E1 (CYP2E1) expression were determined after antioxidants were added to the tBHP-stressed cells. When compared to an ethanol-based control, all biomarkers for the cell damage were significantly increased by treatments. Treatments of β-C or the combination of two antioxidants at 50 ppm for 48 h enhanced cell proliferation (P < 0.05) compared to tBHP control. The antioxidative and cytoprotective actions of -T and β-C, alone or in combination, were associated with modulation of microsomal CYP2E1 expression, corresponding to the regulation of LPO production (P < 0.0001). Our results indicate that -T and β-C may contribute differently to protection of cellular membrane disruption in CYP2E1-expressing HepG2 cells. Moreover, the combination of -T and β-C appears to impel the greater protection of pathogenic processes of oxidative stress in liver.

Key Words: CYP2E1 • DNA adduction • HepG2 • -tocopherol • β-carotene

Toxicology and Industrial Health, Vol. 25, No. 4-5, 311-320 (2009)
DOI: 10.1177/0748233709106443


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