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Transforming growth factor-β3 expression up-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Yue-xian Fu

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Xing Liu

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Lin Qiu

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Sheng-de Wu

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Xiao-fei Tian

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Yan Liu

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

Guang-hui Wei

Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China, ghwei{at}mail.chcmu.com

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate, in which the molecular etiology of the defect is poorly characterized. Recently, transforming growth factor-β3 (TGF-β3) has been indicated to play an essential role in the development of palatal shelves. In this developmental toxicity study, we investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of TGF-β3 in fetal mice. Pregnant C57BL/6 mice were exposed to corn oil or TCDD (32 µg/kg/day 64 µg/kg/day, per os) at embryonic day 10 (ED10), a drastic inhibition of palatal shelves was induced. By using RT-PCR (reverse transcription-polymerase chain reaction) and Western blot, the expressions of TGF-β3 was investigated. We found that the expression of TGF-β3 was gradually up-regulated in TCDD-treated group. These results suggest that cleft palate can be induced by TCDD exposure, the modification of TGF-β3 is related to its pathogenesis.

Key Words: 2 • 3 • 7 • 8-Tetrachlorodibenzo-p-dioxin • cleft palate • transforming growth factor-β3 • mouse

Toxicology and Industrial Health, Vol. 25, No. 7, 473-478 (2009)
DOI: 10.1177/0748233709345940


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