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Toxicology and Industrial Health, Vol. 22, No. 8, 337-341 (2006)
DOI: 10.1177/0748233706071737

Effect of sodium azide on the metabolic activity of cultured fetal cells

Takaki Ishikawa

Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 1-4-3, Abeno, Osaka 545-8585, Japan, takaki{at}med.osaka-cu.ac.jp

Bao-Li Zhu

Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 1-4-3, Abeno, Osaka 545-8585, Japan

Hitoshi Maeda

Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 1-4-3, Abeno, Osaka 545-8585, Japan

Sodium azide is a highly toxic substance. However, the mechanism of its toxicity has not been fully established. In the present study, we attempted to investigate the toxicity of sodium azide in various cultured fetal cells, using changes in cellular respiration as an indicator of metabolic inhibition to elucidate tissue-specificity. The human fetal cell lines used in this study included myocardial cells, nerve cells, fibroblasts, hepatocytes and renal tubular epithelial cells. The cells were seeded in wells at a density of 2 x 106 cells/2mL, sodium azide was added at a concentration of 0.01 ng/mL to 10 mg/mL, and the respiration of each type of cell was measured 1 h later using a dissolved oxygen meter. The concentration at which sodium azide inhibited metabolic activity was lower in the nerve and myocardial cells than in the fibroblasts, hepatocytes and renal tubular epithelial cells. These findings may serve to clarify the dynamic mechanisms of sodium azide toxicity in vivo.

Key Words: cytotoxicity test • mitochondria • oxygen electrode • respiration • sodium azide

References

  • Akanbi, K. A., Brodie, A. E. and Suryawan, A.et al. 1994: Effect of age on the differentiation of porcine adipose stromal-vascular cells in culture . Journal of Animal Science 72, 2828-2835 .[Abstract]
  • Amano, Y., Okumura, C. and Yoshida, M.et al. 1999: Measuring respiration of cultured cell with oxygen electrode as a metabolic indicator for drug screening . Human Cell 12, 3-10 .[Medline] [Order article via Infotrieve]
  • Arai, J., Yamada, K. and Yasuda, M.et al. 1998: Anticancer susceptibility test method using general-purpose dissolved oxygen meter . Human Cell 11, 175-178 .[Medline] [Order article via Infotrieve]
  • Beselt, R. C. 1995: Azide. In Beselt, R. C. editor, Disposition of toxic drugs and chemicals in man, Volume 4. Biomedical Publication , 66-67.
  • Chang, S. and Lamm, S. H. 2003: Human health effects of sodium azide exposure: a literature review and analysis . International Journal of Toxicology 22, 175-186 .[CrossRef][ISI][Medline] [Order article via Infotrieve]
  • Chen, S. J., Bradley, M. E. and Lee, T. C. 1998: Chemical hypoxia triggers apoptosis of cultured neonatal rat cardiac myocytes: modulation by calcium-regulated proteases and protein kinases . Molecular and Cell Biochemistry 178, 141-149 .
  • Edmonds, O. P. and Bourne, M. S. 1982: Sodium azide poisoning in five laboratory technicians . British Journal of Industrial Medicine 39, 308-309 .[ISI][Medline] [Order article via Infotrieve]
  • Ellenhort, M. J. 1988: Sodium azide. In Ellenhorn, M. J., editor, Diagnosis and treatment of human poisoning. Williams and Wilkins , 1385-1388.
  • Feril, L. B. Jr., Tsuda, Y. and Kondo, T.et al. 2004: Ultrasound-induced killing of monocytic U937 cells enhanced by 2,2?-azobis(2-amidinopropane) dihydrochloride . Cancer Science 95, 181-185 .[CrossRef][Medline] [Order article via Infotrieve]
  • Gallager, D. W., Mallorga, P. and Oertel, W.et al. 1981: Diazepam binding in mammalian central nervous system: a pharmacological characterization . Journal of Neuroscience 1, 218-225 .[Abstract]
  • Gao, F., Gong, B. and Christopher, T. A. 2001: Anti-apoptototic effect of benidipine, a long-lasting vaso-dilating calcium antagonist, in ischemic/reperfused myocardial cells . British Journal of Pharmacology 132, 869-878 .[CrossRef][ISI][Medline] [Order article via Infotrieve]
  • Hansen, C., Christensen, P. and Stryhn, H.et al. 2002: Validation of the FACSCount AF system for determination of sperm concentration in boar semen . Reproduction in Domestic Animals 37, 330-334 .[CrossRef][ISI][Medline] [Order article via Infotrieve]
  • Howard, J. D., Skogerboe, K. J. and Case, G. A.et al. 1990: Death following accidental sodium azide ingestion . Journal of Forensic Science 35, 193-196 .
  • Ingraham, H. J., Perry, H. D. and Donnenfeld, E. D. 1991: Air-bag keratitis . New England Journal of Medicine 324, 1599-1600 .[ISI][Medline] [Order article via Infotrieve]
  • Ishikawa, T., Kaku, K. and Miyaishi, S.et al. 2003: Effects of therapeutic on cellular respiration as an indication of metabolic activity . Japanese Journal of Legal Medicine 58, 80-80 .
  • Ishikawa, T., Miyaishi, S. and Tachibana, T.et al. 2004: Fatal hypothermia related vacuolation of hormone-producing cells in the anterior pituitary . Legal Medicine 6, 157-163 .[Medline] [Order article via Infotrieve]
  • Ishikawa, T., Zhu, B.-L. and Maeda, H. 2006: Effects of therapeutic agents on cellular respiration as an indication of metabolic activity . Human and Experimental Toxicology 25, 135-140 .[Abstract/Free Full Text]
  • Kozlocka-Gajdzinska, H. and Brzyski, J. 1966: A case of fatal intoxication with sodium azide . Archives of Toxicology 22, 160-163 .[CrossRef]
  • Marquet, P., Clement, S. and Lotfi, H.et al. 1996: Analytical findings in a suicide involving sodium azide . Journal of Analytical Toxicology 20, 134-138 .[ISI][Medline] [Order article via Infotrieve]
  • Olson, K. R. 1999: Poisoning and drug overdose, Volume 3. Appleton & Lange , 99-101.
  • Richardson, S. G. N. and Giles, C. 1995: Two cases of sodium azide poisoning by accidental ingestion of Isoton . Journal of Clinical Pathology 28, 350-351 .
  • Tabei, I., Hashimoto, H. and Ishiwata, I.et al. 2003: New approach for the establishment of an hepatocyte cell line derived from rat early embryonic stem cells . Human Cell 16, 39-46 .[Medline] [Order article via Infotrieve]
  • Uesu, K. and Ishikawa, H. 2005: Analysis of an in vitro susceptibility test of anticancer drugs using new types oxygen electrodes . Bulletin of Education and Research, Nihon University School of Dentistry at Matsudo 7, 7-21 .
  • Yao, K., Sato, H. and Ina, Y. 2000: Benidipine inhibits apoptosis during ischemic acute renal failure in rats . The Journal of Pharmacy and Pharmacology 52, 561-568 .[CrossRef][ISI][Medline] [Order article via Infotrieve]

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This Article
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